Dear Phil,
We published two papers back in 1982 (oldies but goodies!) showing how
viral binding, including kinetics, could be performed using fluorescent dyes
that partition into the virus. Those particular dyes are probably not
commercially available, but there are a number of other fluorescent
membrane-partitioning dyes that should work. We're not doing that line of
work anymore so I can't give you more recent information. You do need to
test to see that the dyes you use will not interfere with either the binding
or the subsequent biological action that you are studying. Here are the two
references which should give you some help in setting up your studies:
Leary, J.F., Notter, M.F.D.: "Kinetics of Virus Adsorption to Single Cells
Using Fluorescence Membrane Probes and Multiparameter Flow Cytometry" Cell
Biophysics 4: 63-76 (1982).
Notter, M.F.D., Leary, J.F., Balduzzi, P.C.: "Adsorption of Rous
Sarcoma Virus to Genetically Susceptible and Resistant Chick Cells - A Laser
Flow Cytometry Study" J Virology 41: 958-964 (1982).
I hope this helps.
Jim Leary
On 3/16/99 Phillip Barren wrote:
FLOWers
I am trying to look at the binding of virus to cells. Does anyone
have any thoughts on doing this without using antibody to label the virus??
I want to effect the virus/cell binding as little as possible.
Thank you for your time
Philip Barren
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