Too bad I can't be on this one... (I'd like a bit of the champagne) But I'd bet on Calman too, however it would be good if we could collect these different "cookbook" recicpies and publish them on the web somewhere, so that everybody who wants can have a look and choose the protocol that appeals most.... just an suggestion of course... Cheers, Matthias _____________________________________ Dr. Matthias Haury Instituto Gulbenkian de Ciencia Unidade de Imagiologia Celular Rua Da Quinta Grande N° 6 - Apt.14 P-2781 Oeiras Codex Portugal Email: mhaury@igc.gulbenkian.pt Tel: + 351 (01) 440 79 85 Office Tel: + 351 (01) 440 79 10 Secretary Fax: + 351 (01) 440 79 70 _____________________________________ At 2:47 PM -0800 3/16/99, Mario Roederer wrote: >>I will be happy to go hand to hand at the upcoming FASEB meeting with any of >>your technical staff examining Ag specific responses by flow and will bet a >>case of Champagne (OK, 1/2 a case) that my "notoriously variable cookbook" >>system will have less noise and as good a signal as the out of the box "fix >>and perm" kit from Caltag. Are we on? Kevin Holmes can be the referee, >>results to reported to the Cytometry mailing list. > >Cool! I'm in on this one! Let's up the ante, and make it a full case of >Roederer champagne (i.e., the good stuff). I'll bet on Calman. > >(By the way, we use "cookbook" methods that are, by today's standards, >quite dated--and they still give us excellent results that are >reproducible. We use PharMingen antibodies and home-made fix/perm >solutions). > >mr
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