Re: Virus detection

From: Daryl Webb (dwebb@schooner.waite.adelaide.edu.au)
Date: Tue Mar 16 1999 - 22:39:38 EST


According to Barren, Phil:
> 	I am trying to look at  the binding of virus to cells. Does anyone
> have any thoughts on doing this without using antibody to label the virus??
> I want to effect the  virus/cell binding as little as possible.

Phil, In a previous life i did some binding studies of Chlamydia 
trachomatis (sp) EB and RB's to T and B cells.
basically we allowed the binding to occur
fixed the cells and bacteria
stained with antibody to cell + chlamydia

I dont know if this would solve your problem but it worked for us.

(i have a reference buried somewhere if you need it)

best of luck

-- 

  Daryl Webb   (dwebb@waite.adelaide.edu.au)
  Dept. of Plant Science,  Waite Institute
  University of Adelaide,  Glen Osmond S.A. 5064
  Australia. Voice:61_8 8303 7392  Fax:61_8 8303 7102

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