Dear Lee, We developed a very fast-automated whole blood lysing technique based on an inorganic-acid. In this method lysing of the red cells is established by a very short pH-shock. A disadvantage of this technique is that we have not been able to use a buffer like PBS for the dilution of whole blood. We tested a lot of alternatives and found very good results with Alsevers solution (Sigma, product number A-3551, contains 4.2g NaCl, 8.0g sodium citrate and 20.5g glucose/L). When diluting in this solution we have even seen an improvement of the morphology and antigenicity over the not diluted whole blood. I hope this tip is of some value for you, if you would like to learn more about our lysing technique I can advise you to have a look at our web site: www.clb.nl <http://www.clb.nl> . Best regards, John Voorn Product manager CLB Reagents tel: +31-20-5123246 Plesmanlaan 125 fax: +311-20-5123570 1066CX Amsterdam E-mail: J_Voorn@CLB.nl <mailto:J_Voorn@CLB.nl> the Netherlands Site: WWW.CLB.nl <http://WWW.CLB.nl>
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