>I mainly uses the cytometry for immunophenotyping of whole blood or bone >marrow samples for haematological diseases identification (e.g. >leukaemia, CLPD). Before the staining of blood with clones, I will >adjust the cell concentration to a desirable count. I currently use the >self-prepared pH 7.4 PBS with 10% albumin for dilution. >1. Does anyone use other type of dilutent? >2. Do interferences occur if the dilutent contains preservative e.g. >Coulter IsoFlow? My personal preference is for a culture medium of some sort e.g RPMI - it keeps the cells stable for a longer time. Te-Chih Liu NUH Haematology Singapore
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