I am having no luck in my attempt to synchronize EL4 and S49 (both T cell lines) cells in either G0, G1, or early S phase. I have attempted ILE depravation, aphidicholine treatment, and varying times in Mimosene, or hydroxyurea. The best I could accomplish without killing all of my cells was a 16 hour incubation period in either mimosene or hydroxyurea, which resulted in about 60% of cells blocked at the appropriate stage as measured by PI. I was hoping I could do a lot better than that, and was wondering if anyone in this list has experience in synchronization of T cell lines that leaves the cells viable for experimentation upon release. Thanks to all in advance. Cathy Miller C581812@showme.missouri.edu
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:10 EST