Maybe coat with collagen also? > ---------- > From: Moshe Kushnir > Sent: Friday, February 5, 1999 3:34 AM > To: Cytometry Mailing List > Subject: Some like it hot > > > Dear members of the list: > Here's a somewhat peculiar request for info: > I am aware that for the flow community a most common problem is how to > keep cells apart. I have a problem with keeping them together. > I grow P19 cells in monolayers for a confocal study using fluorescent > dyes. It is imperative that the monolayers reach confluency for reasons of > quantization during the experiment. However, within a few hours of > reaching confluency P19 monolayers tend to detach and fold at the > slightest manipulation. My questions are: does any of you know of a way to > make the monolayers adhere more strongly to the bottom of the culture > dishes/well clusters? Would precoating with a matrix substance > (fibronectin?) or polylysine help? Are there commercially available > precoated culture dishes/clusters? > Any input will be appreciated. > > Moshe Kushnir, PhD > mkushnir@playfair.utoronto.ca > Playfair Neuroscience Unit > Toronto Western Hospital > 399 Bathurst st. > Toronto, ON M5T 2S8 > Canada > >
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