Many thanks to the members of the list replying to my question re monolayer adherence. Here's a brief summary for the benefit of future members encountering the same problem: 1. Coating culture dishes with fibronectin, collagen, laminin, polylysin etc., may be instrumental in helping with this problem. 2. Other treatments commercially available like Cell+ treated culture ware, Cell-taq etc. may be of use as well. I am currently checking Cell+ dishes from Sarstedt, and will report results shortly. Again, thanks to Deb Berglund, Patricia Echeagaray, Yuyu Pryor, "Janette", Barbara Butcher, Basliel Gabriel and Julia Philips. MK
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