Dear members of the list: Here's a somewhat peculiar request for info: I am aware that for the flow community a most common problem is how to keep cells apart. I have a problem with keeping them together. I grow P19 cells in monolayers for a confocal study using fluorescent dyes. It is imperative that the monolayers reach confluency for reasons of quantization during the experiment. However, within a few hours of reaching confluency P19 monolayers tend to detach and fold at the slightest manipulation. My questions are: does any of you know of a way to make the monolayers adhere more strongly to the bottom of the culture dishes/well clusters? Would precoating with a matrix substance (fibronectin?) or polylysine help? Are there commercially available precoated culture dishes/clusters? Any input will be appreciated. Moshe Kushnir, PhD mkushnir@playfair.utoronto.ca Playfair Neuroscience Unit Toronto Western Hospital 399 Bathurst st. Toronto, ON M5T 2S8 Canada
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