Yes, you can coat with fibronectin, collagen or laminin, any should help. I grow cells on glass coverslips, each pretreated with 50 ul of a 1mg/ml solution (in water) of laminin. You can buy coated dishes, we don't so I have no recommendations. Also your cells will adhere best if you maintain Ca and Mg ions in your labeling and wash solutions. Also do it quickly, gently and on ice. Good luck, let me know if you want more friendly advice. Deb Berglund Montana State University On Fri, 5 Feb 1999, Moshe Kushnir wrote: > > Dear members of the list: > Here's a somewhat peculiar request for info: > I am aware that for the flow community a most common problem is how to > keep cells apart. I have a problem with keeping them together. > I grow P19 cells in monolayers for a confocal study using fluorescent > dyes. It is imperative that the monolayers reach confluency for reasons of > quantization during the experiment. However, within a few hours of > reaching confluency P19 monolayers tend to detach and fold at the > slightest manipulation. My questions are: does any of you know of a way to > make the monolayers adhere more strongly to the bottom of the culture > dishes/well clusters? Would precoating with a matrix substance > (fibronectin?) or polylysine help? Are there commercially available > precoated culture dishes/clusters? > Any input will be appreciated. > > Moshe Kushnir, PhD > mkushnir@playfair.utoronto.ca > Playfair Neuroscience Unit > Toronto Western Hospital > 399 Bathurst st. > Toronto, ON M5T 2S8 > Canada > >
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