Colleagues, My question concerns the Phase-Resolved flow cytometry paper by Jan Keij et al. in the january Cytometry. I had thought people were routinely utilizing Hoechst/MCB to examine glutathione and relative DNA content and had hoped to shortly do this myself on our FACStar Plus utilizing UV excitation and collection across a 440LP dichroic to a standard FITC filter fot the MCB and a 450/50 for the Hoechst. Is monobromobimane much more specific for GSH and its overlapping emission profile relative to Hoechst the central issue here? Gene/UCONN Health
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