Fran Gibson wrote : >Am I right in thinking that the line Hut78 constitutively expresses >Fas-L and Jurkats express Fas. More precisely their Fas expression levels are (n=3, CV~10%): JURKAT : 19,000 Fas sites/cell HUT78 : 130,000 Fas sites/cell In addition, MOLT4 express 16,000 Fas sites/cell (at least for the cells we have in our own lab). No data yet fot FasL. > Will the same reagents upregulate Fas expression ? Tricks available if you plan to quantitate Fas expression on PBMC on an absolute (i.e. reproducible) basis. Do not hesitate to contact us. Good luck! Philippe Poncelet Director R&D BioCytex -----Message d'origine----- De : frances gibson <fgibson@sghms.ac.uk> À : Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> Date : mercredi 6 janvier 1999 18:33 Objet : Fas and Fas-L > >Thinking about positive controls for surface Fas and Fas-L expression >by flow. >Am I right in thinking that the line Hut78 constitutively expresses >Fas-L and Jurkats express Fas. >Also what will upregulate Fas-L on PBMCs ? PHA, PMA and/or ionomycin ? >Are these reagents acting on different cell types ie. T cells versus >monocytes ? Will the same reagents upregulate Fas expression ? > >Thanks for any help. > >Fran Gibson >Dept Haematology >St George's Hospital Medical School >London >fgibson@sghms.ac.uk > > >
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