Hi Doug,
Do not hesitate a minute. It's fun to play with platelets!
We even do surface glycoproteins quantitation on them. Easy but :
- prefer PRP for your 1st time, then go to whole blood (quicker);
- prefer citrated blood and store at room temperature rather than in the
cold ;
- prefer no-wash IF protocols (we use standardized whole-blood, no-wash
indirect IF protocols every day)
- prefer log FS x log SS rather than linear for dual scatter plot (easier to
find platelet cloud);
- be sure to clean your sample tubing after other type of samples (Clenz or
equivalent);
- have a 0.22µm sheath filter on-line;
- .... so many tricks .... Contact us if you need more infos.
Don't worry , be happy !
Philippe Poncelet
BioCytex
-----Message d'origine-----
De : Douglas S. Smoot <dsmoot@med.navy.mil>
À : Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
Date : mercredi 6 janvier 1999 18:12
Objet : Platelets
>
>Today I was asked about running platelets on my flow cytometer. I've never
>run them, so are there any special tricks that I should be aware of? I
have
>a Coulter Elite, if that makes a difference. Thank you.
>
>Doug
>{-----------------------------------------------------------------------}
>Douglas Smoot
>Immune Cell Biology Program
>Naval Medical Research Center
>8901 Wisconsin Avenue
>Bethesda, MD 20889-5607
>voice: 301-295-6272
>fax: 301-295-0376
>
>
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