Thinking about positive controls for surface Fas and Fas-L expression by flow. Am I right in thinking that the line Hut78 constitutively expresses Fas-L and Jurkats express Fas. Also what will upregulate Fas-L on PBMCs ? PHA, PMA and/or ionomycin ? Are these reagents acting on different cell types ie. T cells versus monocytes ? Will the same reagents upregulate Fas expression ? Thanks for any help. Fran Gibson Dept Haematology St George's Hospital Medical School London fgibson@sghms.ac.uk
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