I am posting this reply to the list for Craig Jordan please send any comments directly to him at :cjordan@pop.uky.edu >Mime-Version: 1.0 >Date: Tue, 20 Jan 1998 10:57:29 -0500 >From: "Craig T. Jordan, PhD" <cjordan@pop.uky.edu> >Subject: Re: CD34 negative stem cells > >Please keep in mind that the results of Goodell were only functionally >proven in mice. There is as yet no formal proof that human stem cells can >be CD34 dim to negative. Moreover, even if a more primitive cell than the >conventional phenotype does exist, it does not change the fact that the >CD34+ subset contains a transplantable multi-lineage cell. Thus, we feel >it is still useful to work with the "old" stem cell phenotype. >Craig T. Jordan,PhD >Markey Cancer Center >800 Rose St. Room CC407 >Lexington, KY >40536-0093 >606-323-5688 (phone) >606-257-7715 (fax) >606-323-3000, #3330 (pager) > >>>Date: Fri, 16 Jan 1998 21:09:49 -0500 >>>From: Keith Bahjat <kbahjat@ufl.edu> >>>Reply-To: kbahjat@ufl.edu >>>MIME-Version: 1.0 >>>To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> >>>Subject: CD34 negative stem cells >>> >>> >>>In reference to the recent article: >>> >>>Goodell M et al. "Dye efflux studies suggest that hematopeitic stem >>>cells expressing low or undetectable levels of CD34 antigen exist in >>>multiple species"; Nature Medicine Vol.3 No.12, December 1997. >>> >>>For the stem cell experts amongst us, how do feel this impacts the >>>thinking when performing either stem cell purification (for autologous >>>reconstitution) or stem cell enumeration? As these cells are more >>>primitive than the CD34+ stem cell, should we infer that they may be >>>better suited to hematopoietic reconstitution? >>> >>>Thanks for any thoughts you'd like to share. >>> >>>-- >>>Keith Bahjat >>>Graduate Assistant >>>University of Florida >>>College of Medicine >>>Gainesville, Florida >>>kbahjat@ufl.edu >>>
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