Dear Cynthia, A lot of really nice work has been done to explore murine stem cells by Ken Shortman, R. Scollay and collaborators. They published several papers in the early 1990s including one entitled "A simplified method for enrichment of mouse hematopoietic stem cell" Exp. Hematol 18:920, 1990 by Spandgrude, G. J. and Scollay, R. that may be of interest to you. In general, people exploring murine pluripotent stem cells look for cells that are lineage marker (Lin-) negative, c-kit (CD117)+, and Sca1+. Various other methods/markers identifying these cells have been utilized as well. Irving Weissman's group recently published a paper in Cell identifying a lymphoid committed progenitor in bone marrow, and I know they have published other papers in the past dealing with this subject. I would recommend doing a medline search on any of these individuals and that will give you a place to start. Good luck! Noelle S. Williams Department of Pathology UT Southwestern Medical Center 6000 Harry Hines Boulevard Dallas, TX 75235-9072 (214)-648-4081 (W)
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