>From owner-cyto-sendout@flowcyt.cyto.purdue.edu Fri Jan 2 15:58:22 1998 >Received: by flowcyt.cyto.purdue.edu (940816.SGI.8.6.9/930416.SGI.AUTO) > for cyto-sendout id RAA29818; Fri, 2 Jan 1998 17:30:02 -0500 >Received: from mx.afrri.usuhs.mil by flowcyt.cyto.purdue.edu via ESMTP (940816.SGI.8.6.9/930416.SGI.AUTO) > for <cytometry@flowcyt.cyto.purdue.edu> id IAA29053; Fri, 2 Jan 1998 08:55:05 -0500 >Received: from wyant.usuhs.mil by mx.afrri.usuhs.mil (Unoverica 2.11a) > id 000013DC; Fri, 2 Jan 1998 08:57:45 -0500 >Message-Id: <3.0.1.32.19980102085356.00694bd4@mx.afrri.usuhs.mil> >X-Sender: Wyant@mx.afrri.usuhs.mil >X-Mailer: Windows Eudora Light Version 3.0.1 (32) >Date: Fri, 02 Jan 1998 08:53:56 -0500 >To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> >From: Cynthia Wyant <Wyant@mx.afrri.usuhs.mil> >Subject: bone marrow >Mime-Version: 1.0 >Content-Type: text/plain; charset="us-ascii" > > >I am looking for help with analyzing mouse bone marrow for primitive myloid >and lymphoid progenitors with FACS caliber. Specifically is there a >population within the total bone marrow that I need to focus on to optimize >my instrument and to locate stem cells? Are there any texts or >instructional papers available on this subject? Is anyone in the Virginia, >Maryland, D.C. area working with mouse bone marrow and, if so, would you be >willing to let me visit your lab? I would love to see first hand your >methods for manipulating bone marrow in the flowcyotmeter. > >Cyndi Wyant >wyant@mx.afrri.usuhs.mil >Armed Forces Radiobiology Research Institute >Bethesda, MD > Cyndi, Check out this paper, I think it might help you. "Expression of Murine CD38 Defines a Population of Long-Term Reconstituting Hematoppoietic Stem Cells" Troy Randall, Blood, Vol 87, No 10 (May 15), 1996: pp4057-4067 If this doesn't help, I have Dr Randall's e-mail addy, and he may be able to answer your questions. Hope this helps, Bill Nostrom Trudeau Institute Saranac Lake, NY ______________________________________________________ Get Your Private, Free Email at http://www.hotmail.com
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