Cyndi In addition to Bill's suggestion, a paper characterising the RAM34 antibody (which identifies "murine CD34") may be worth looking at: Primitive hematopoietic cells in murine bone marrow express the CD34 antigen. F Morel, SJ Szilvassy, M Travis, B Chen, A Galy. Blood 88(10) 1996 3774-84 also the use of phenotype such as Sca1+, c-kit+, Thy1.1lo, lin-: (one example from many) The unexpected G0/G1 cell cycle status of mobilized hematopoietic stem cells from peripheral blood. N Uchida, D He, AM Friera, M Reitsma, D Sasaki, B Chen, A Tsukamoto. Blood 89 (2) 1997 465-472 and a method using dual wavelength analysis of Hoechst 33342 (I realise this can not be done on the FACSCalibur, but interesting just the same): Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo. MA Goodell, K Brose, G Paradis, AS Conner, RC Mulligan. J Exp Med 1996 183 1797-1806. Dye efflux studies suggest that hematopoietic stem cells expressing low or undetectable levels of CD34 antigen exist in multiple species. MA Goodall, M Rosenzweig, H Kim, DF Marks, MA DeMaria, G Paradis, SA Grupp, CA Sieff, RC Mulligan, RP Johnson. Nature Medicine 1997 3 (12) 1337-1345. Ian Titley PhD Leukaemia Research Fund Centre at the Institute of Cancer Research 237 Fulham Road LONDON SW3 6JB UK Tel: +44 171 352 8133 Fax: +44 171 352 3299 E-mail: iant@icr.ac.uk On Mon, 05 Jan 1998 08:13:29 PST william nostrom <slickwilli@hotmail.com> wrote: > > > > >From owner-cyto-sendout@flowcyt.cyto.purdue.edu Fri Jan 2 15:58:22 > 1998 > >Received: by flowcyt.cyto.purdue.edu (940816.SGI.8.6.9/930416.SGI.AUTO) > > for cyto-sendout id RAA29818; Fri, 2 Jan 1998 17:30:02 -0500 > >Received: from mx.afrri.usuhs.mil by flowcyt.cyto.purdue.edu via ESMTP > (940816.SGI.8.6.9/930416.SGI.AUTO) > > for <cytometry@flowcyt.cyto.purdue.edu> id IAA29053; Fri, 2 Jan 1998 > 08:55:05 -0500 > >Received: from wyant.usuhs.mil by mx.afrri.usuhs.mil (Unoverica 2.11a) > > id 000013DC; Fri, 2 Jan 1998 08:57:45 -0500 > >Message-Id: <3.0.1.32.19980102085356.00694bd4@mx.afrri.usuhs.mil> > >X-Sender: Wyant@mx.afrri.usuhs.mil > >X-Mailer: Windows Eudora Light Version 3.0.1 (32) > >Date: Fri, 02 Jan 1998 08:53:56 -0500 > >To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > >From: Cynthia Wyant <Wyant@mx.afrri.usuhs.mil> > >Subject: bone marrow > >Mime-Version: 1.0 > >Content-Type: text/plain; charset="us-ascii" > > > > > >I am looking for help with analyzing mouse bone marrow for primitive > myloid > >and lymphoid progenitors with FACS caliber. Specifically is there a > >population within the total bone marrow that I need to focus on to > optimize > >my instrument and to locate stem cells? Are there any texts or > >instructional papers available on this subject? Is anyone in the > Virginia, > >Maryland, D.C. area working with mouse bone marrow and, if so, would > you be > >willing to let me visit your lab? I would love to see first hand your > >methods for manipulating bone marrow in the flowcyotmeter. > > > >Cyndi Wyant > >wyant@mx.afrri.usuhs.mil > >Armed Forces Radiobiology Research Institute > >Bethesda, MD > > > > Cyndi, > Check out this paper, I think it might help you. > > "Expression of Murine CD38 Defines a Population of Long-Term > Reconstituting Hematoppoietic Stem Cells" Troy Randall, Blood, Vol 87, > No 10 (May 15), 1996: pp4057-4067 > > If this doesn't help, I have Dr Randall's e-mail addy, and he may be > able to answer your questions. > > Hope this helps, > Bill Nostrom > Trudeau Institute > Saranac Lake, NY > > > > > ______________________________________________________ > Get Your Private, Free Email at http://www.hotmail.com
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