sorting live yeast

From: Rochelle A. Diamond (diamond@cco.caltech.edu)
Date: Tue Jan 06 1998 - 18:37:46 EST

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Hi Flow Community-
I would like to get some information about sorting live yeast cells.  

In particular I would like to know the following:
	How badly do they reaggregate after filtering thru nylon mesh ( do
I need to worry about the size of my orifice?)
	How do you decontaminate the sorter after the run (preferred
cleansing agents)?
	What kind of autofluorescence do yeast cells (S. Cere.) exhibit?
	Any experiences good or bad would be useful.

The experiment is to sort out high and low expressing GFP (EGFP-like)
cells.

Thanks ever so much
Rochelle Diamond
Caltech Biology
Flow Cytometry/Cell Sorting Facility
diamond@cco.caltech.edu

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