Hello Stefan, We have a number of customers using the MoFLo to analyze and sort bacteria at high-speed. I wanted to reply to some of the points brought up by Howard about the MoFlo's utility in that regard. We have in fact delivered a number of MoFLo's with forward scatter PMT's. Our customers primarily use this as a forward fluorescence detector, rather than a light scatter detector, for sperm sorting. It is capable of being used for light scatter. For microbial applications we have found that sorting is best accomplished with a 90 degree log threshold when dealing with unlabeled material, otherwise fluorescence trigger. Forward scatter triggering can get messy when using small particles since droplet drive can show up in that detector. In response to the question of moving lasers from position one to two , when using the MoFLo it is very simple since beam steering optics are all independent. The beams are simply steered to the appropriate pinhole. It takes less than 5 minutes. Peter Lopez ------------------------------------------------------------------------ ---------------------------------------------------------- Peter A. Lopez 970.226.2200 Cytomation,Inc. 400 E. Horsetooth Rd. Ft. Collins,CO USA Manager, Applications Laboratory PeterL@cytomation.com http://www.cytomation.com ------------------------------------------------------------------------ -----------------------------------------------------------
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