FCM for natural bacteria

From: Peter Lopez (Peterl@cytomation.com)
Date: Tue Nov 25 1997 - 11:27:28 EST


Hello Stefan,

	We have a number of customers using the MoFLo to analyze and
sort bacteria at high-speed. I wanted to reply to some of the points
brought
up by Howard about the MoFlo's utility in that regard. 
	We have in fact delivered a number of MoFLo's with
forward scatter PMT's. Our customers primarily use this as a forward
fluorescence
detector, rather than a light scatter detector, for sperm sorting. It is
capable of being used for light scatter. 
	For microbial applications we have found that sorting is best
accomplished
with a 90 degree log threshold when dealing with unlabeled material,
otherwise fluorescence trigger. Forward scatter triggering can get messy
when using small particles since droplet drive can show up in that
detector.
	In response to the question of moving lasers from position one
to two , when
using the MoFLo it is very simple since beam steering optics are all
independent. The
beams are simply steered to the appropriate pinhole. It takes less than
5 minutes.

Peter Lopez


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Peter A. Lopez                                         970.226.2200
Cytomation,Inc.                                        400 E. Horsetooth
Rd. Ft. Collins,CO USA
Manager, Applications Laboratory            PeterL@cytomation.com
                               http://www.cytomation.com
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