> Hello Stefan, > > We have a number of customers using the MoFLo to analyze and > sort bacteria at high-speed. I wanted to reply to some of the points > brought > up by Howard about the MoFlo's utility in that regard. > We have in fact delivered a number of MoFLo's with > forward scatter PMT's. Our customers primarily use this as a forward > fluorescence > detector, rather than a light scatter detector, for sperm sorting. It > is capable of being used for light scatter. > For microbial applications we have found that sorting is best > accomplished > with a 90 degree log threshold when dealing with unlabeled material, > otherwise fluorescence trigger. Forward scatter triggering can get > messy when using small particles since droplet drive can show up in > that detector. > In response to the question of moving lasers from position one > to two , when > using the MoFLo it is very simple since beam steering optics are all > independent. The > beams are simply steered to the appropriate pinhole. It takes less > than 5 minutes. > > Peter Lopez > > > ---------------------------------------------------------------------- > ------------------------------------------------------------ > Peter A. Lopez 970.226.2200 > Cytomation,Inc. 400 E. > Horsetooth Rd. Ft. Collins,CO USA > Manager, Applications Laboratory PeterL@cytomation.com > http://www.cytomation.com > ---------------------------------------------------------------------- > ------------------------------------------------------------- >
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