Salut tout le monde... Does anybody of you have experience of using the TUNEL technique to test apoptosis in mouse thymocytes directly ex vivo (no incubation in culture) ? I'm just curious if one can detect the apopotosis of double positive thymocytes directly, or if a incubation in culture is necessary to see anything... I know that this subject has been discussed before, but it's very hard to search the archive from here, as the network connections are terribly jammed in the moment... Thanks a lot again, Matthias _____________________________________________________________________________ Matthias Haury Flowcytometry Dept Immunology Institut Pasteur mhaury@pasteur.fr Tel: 33 (01) 40 61 31 29 Fax: 33 (01) 45 68 86 39 _____________________________________________________________________________ƒ
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