--- On Fri, 24 Oct 1997 16:19:43 +0200 Matthias Haury <mhaury@pasteur.fr> wrote: > >Does anybody of you have experience of using the TUNEL technique to test >apoptosis in mouse thymocytes directly ex vivo (no incubation in culture) ? > >I'm just curious if one can detect the apopotosis of double positive >thymocytes directly, or if a incubation in culture is necessary to see >anything... We have never looked at mouse thymocytes, but it is my understanding that for most cell systems, the in vivo methods of removal of apoptotic cells are so efficient that they are very difficult to detect in the absence of an incubation period. If they were detectable, an assay which picked up an earlier event may have a better chance of succeeding, such as mitochondrial alteration or phosphatidylserine exposure. Good luck, Tom -------------------------------------------------------- Thomas W. Mc Closkey, Ph. D. Director, Flow Cytometry North Shore University Hospital Biomedical Research Center 350 Community Drive Manhasset, Long Island, New York 11030 ph: 516-562-4844 [office]; 516-562-1135/4641 [lab] 10/27/97 1:56:12 PM E-mail: thomasm@nshs.edu --------------------------------------------------------
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