Identification of Eosinophils in Flow Cytometry

From: Hoffmann, H. J. (hjh@post1.tele.dk)
Date: Tue Jun 17 1997 - 09:41:35 EST 

We work (almost) excliusively with human Eosinophils, and analyse 
them on our Coulter Epics Profile II.  Mostly, we purify them and do 
not need to be able to identify them amongst other cells on a FS-SS 
plot, which is difficult on our machiine, as they lie in the same 
region as neutrophils.

I have recently dealt with someone that runs a BD FACS, and claims to 
be able to identify Eosinophils as a population discrete from 
neutrophils.

As we are considering purchase of a new flow cytometer, I would like 
to invite comments on the identification of eosinophils on different 
flow cytometers.

thanks very much!
hjh
Hans Juergen Hoffmann - Lung Research Clinic
    Aarhus University Hospital, Denmark

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