Eosinophils are readily identifiable in most leucocyte preps. They run as a discrete subpopulation (on a BD FACSort) in a forward-scatter (X) versus side-scatter plot (Y), above the neutrophils and maybe just slightly behind them (don't turn the side-scatter gain too high, or you'll lose them out the top of your plot). Also, if you draw a gate around them and look at this in any fluorescence channel you'll find them to be more autofluorescent than other leucocytes. Robin Barclay BTS Edinburgh
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