Hello,
I'd like to back up Howard Shapiro's comments on the usefulness of
polarised light in the detection of eosinophils, which he recommended to us
some years ago. We routinely use this method combined with forward and side
scattered light for the detection of murine eosinophils from lavage and
peritoneal washings. In stimulated mice, eosinophils can represent up to 60%
of the total population and have a much lower side scatter than in human
samples, and a forward scatter similar to lymphoid cells. Initially we found
these scatters hard to believe but subsequent sorting, staining and
microscopic investigations have shown that it really is the case.
For an example, point your WWW browser at
http://jcsmr.anu.edu.au/facslab/eos.html
Geoff
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Geoffrey Osborne | ____ __ o Ahh!
Flow Cytometry (FACS LAB) | __ `\ <,_
John Curtin School of Medical Research, | __ (*)/ (*)
Australian National University, | ==============|
CANBERRA, AUSTRALIA. | |--|
Email: Geoff.Osborne@anu.edu.au | |--|...
Phone: 61 6 249 3694
FAX: 61 6 249 2595
-----Surfing the Web?: Try http://jcsmr.anu.edu.au/facshome.html------
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