bulk removal of dead cells

From: Ralph Bohmer (ralph.bohmer@es.nemc.org)
Date: Thu Jun 19 1997 - 09:15:33 EST 

Is there any perfect method to bulk isolate a small number of vital cells 
from a much larger population of dead and dying cells (apoptotic, necrotic, 
debris) ?

It would have to be quantitative.

There is lysis of dead cells by protease. Does that safely preserve all 
viable cells ? If yes, what are the best conditions ?

I was told it can be done by density gradient, but I cannot figure out how 
that should work.

Many thanks for your opinions.

Ralph M. Bohmer

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