>Dear Group, > >An investigator wants to look (using flow) at Ca++ responses that occur >within seconds (as in 1 or 2 seconds) after the addition of a particular >agent to dissociated endothelial/epithelial cells. Can anyone tell us >the fastest transit time from sample uptake to laser intercept >with either a Coulter Elite or FACSVantage? I also seem to remember a >commercially available device that could be attached to (any?) flow >cytometer and that increased the speed of sample injection. Does anyone >know the particulars about this device - manufacturer, cost, and how well >it works? Can it can be attached to both the Coulter Elite and the >FACS Vantage? > >Thanks for any info. > >Ray > >++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ >Raymond B. Hester, Ph.D. >Flow Cytometry Lab >CSAB 357, Biotechnical Services >The University of South Alabama >Mobile, AL 36688-0002 To err is human - >Email: rhester@jaguar1.usouthal.edu but it feels divine. >Voice: (334) 460-6029 Mae West >FAX: (334) 460-6073 >++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ The item you are looking for is from Cytek Development in Fremont, California. Details below. Cytek Development 1537 Fulton Place Fremont, CA 94539 Ph: (510) 657-0102 FAX: (510) 657-4309 Product: Time Zero Module Price: $4250.00 (as of 1995) good luck. Keith Bahjat Kbahjat@nwu.edu
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