Re: Transit time

From: LEKAHN@monsanto.com
Date: Fri Mar 07 1997 - 08:59:38 EST


     The device I know about is made by Cytek (510)657-0102.  It is called
     a Time Zero Module and I think it's around $5000.  I have also seen
     some papers in Cytometry over the years with more lab-made devices
     that might work for you.

     Larry Kahn
     Searle Discovery Research


______________________________ Reply Separator _________________________________
Subject: Transit time
Author:  owner-cyto-sendout@flowcyt.cyto.purdue.edu at INTERNET
Date:    3/6/97 6:33 PM


Dear Group,

An investigator wants to look (using flow) at Ca++ responses that occur
within seconds (as in 1 or 2 seconds) after the addition of a particular
agent to dissociated endothelial/epithelial cells.  Can anyone tell us
the fastest transit time from sample uptake to laser intercept
with either a Coulter Elite or FACSVantage?  I also seem to remember a
commercially available device that could be attached to (any?) flow
cytometer and that increased the speed of sample injection.  Does anyone
know the particulars about this device - manufacturer, cost, and how well
it works?  Can it can be attached to both the Coulter Elite and the
FACS Vantage?

Thanks for any info.

Ray

++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Raymond B. Hester, Ph.D.
Flow Cytometry Lab
CSAB 357, Biotechnical Services
The University of South Alabama
Mobile, AL 36688-0002                      To err is human -
Email: rhester@jaguar1.usouthal.edu             but it feels divine.
Voice:



This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:49:31 EST