If you are talking about time taken to remove tube squirt stuff in, replace tube then boost until cells reach laser intercept a well set up operator can do it in about 10 seconds, 9 on a very good day (FACS Vantage). Dont know about Coulters. Cytek make a module that enables very fast squirting without taking the tube off that bolts on to a Vantage, I dont have their phone number to hand. Simon Monard Aaron Diamond Center New York
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