Dear Group, An investigator wants to look (using flow) at Ca++ responses that occur within seconds (as in 1 or 2 seconds) after the addition of a particular agent to dissociated endothelial/epithelial cells. Can anyone tell us the fastest transit time from sample uptake to laser intercept with either a Coulter Elite or FACSVantage? I also seem to remember a commercially available device that could be attached to (any?) flow cytometer and that increased the speed of sample injection. Does anyone know the particulars about this device - manufacturer, cost, and how well it works? Can it can be attached to both the Coulter Elite and the FACS Vantage? Thanks for any info. Ray ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Raymond B. Hester, Ph.D. Flow Cytometry Lab CSAB 357, Biotechnical Services The University of South Alabama Mobile, AL 36688-0002 To err is human - Email: rhester@jaguar1.usouthal.edu but it feels divine. Voice: (334) 460-6029 Mae West FAX: (334) 460-6073 ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
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