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MoFlo OpticsThe foundation of the MoFlo is the industry standard optical bench, which is designed to eliminate vibration. This bench provides an extremely rigid, heavy surface on which the optical and fluidic subsystems are mounted. Each laser path consists of a laser, a Universal Shutter Tower, filters, lenses and dynamic range detectors.Each laser is independently The high precision Universal Shutter Tower combines optimum laser steering with an integral beam-blocking shutter. Controlled by illuminated, color-coded relays, the Universal Shutter Tower provides high efficiency reflectance, wavelength-specific mirrors for each optical pathway. The MoFlo preserves vertical polarization of the laser light, thus ensuring optimum illumination of the sample. All optical elements are of the highest quality and designed for laser optical research or high-resolution microscopy. Spatial and chromatic filtering, with collimating lenses on each laser collection pathway, maximize signal quality and signal-to-noise ratio and minimize inter-laser cross-talk. Only standard 25 mm (0.98 in.) optical filters and dichroic mirrors are used in the MoFlo; no costly special-order filters are required. Hammamatsu photomultiplier tubes Two infrared cameras view the stream. One is positioned to observe the stream emerging from the CytoNozzle™ the other camera views the stream at the last attached drop during sorting. These built-in cameras eliminate the need for tedious viewing microscopes. The second camera is also used by SortMaster™, which automates the determination of the droplet break-off measurement and controls the break-off during the sort. Additional optics information:
For more information about our products, please contact Cytomation at information@cytomation.com.
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steered and focused for easy, stable alignment. Laser focusing is accomplished using either spherical or elliptical illumination optics. High-precision Newport translation stages are used on all optical elements. They deliver sub-micron positioning accuracy and stability as well as minimum hysteresis during adjustment.
were chosen for use with the MoFlo because of their superior performance. Selection from the models available is based on the requirements of the application. A photomultiplier tube may be used in place of the photodiode in the forward light scatter position to facilitate experiments analyzing bacteria, algae and sperm.