MoFlo® Accessories
Elliptical Beam Shaping Optics (EBSO)
The Elliptical Beam Shaping Optics focus the laser beam to an elliptical spot at the inspection point. This reduces cell-to-cell illumination variations and minimizes optical cross-talk.
Superior Energy Distribution
Spherical optics illuminate the core sample stream with a circular energy distribution. This circular energy distribution is not optimal for cells flowing near the edge of the sample stream, especially when running dilute samples at a high differential pressure. With the Elliptical Beam Shaping Optics (EBSO), the energy distribution is tightly focused into an ellipse with an aspect ratio of 1:5 (Figure 1). 
This elliptical energy distribution ensures optimal irradiation of passing particles, thereby lessening the effect of sample pressure on %CV.
Greater Sensitivity With Less Laser Power
The EBSO provide value for cost. By more tightly focusing the energy distribution of the laser, the EBSO achieve the same fluorescent signal intensity while using less laser power. In cases where increased sensitivity is required, the EBSO can provide up to 3.5 times more illuminating laser energy on the sample than traditional spherical optics.
Greater Doublet Discrimination Sensitivity
The EBSO shorten pulse lengths. For experiments using highly concentrated particles or actively dividing cells, these short pulse lengths directly correspond to increased doublet discrimination sensitivity. The EBSO can allow a substantial increase in the resolution of doublets.
Reduction In Cross-Talk
The elliptical energy distribution produced by the EBSO allow the MoFlo to become even more resistant to intra-laser optical cross talk. An 80% reduction in vertical beam size virtually eliminates the potential for fluorescent signal contamination by an adjacent laser. With this improved spatial separation, filter selection becomes easier than ever before.
| Technical Specifications |
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| EBSO includes (for each option): | EBSO module |
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| Focusing lens light tube |
| Chamber light tube |
| EBSO Options: | 351,488, 488A and 633 nm (see below for details) |
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| Typical Ellipse Aspect Ratio: | 1:5 (vertical:horizontal) |
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| Typical Footprint: | 15 x 60 µm |
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| EBSO Options: | Associated Lasers |
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| 351 nm: | Large-frame, water-cooled Argon-ion lasers with beam diameter between 1.4 and 1.7 mm |
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| 488 nm: | Large-frame, water-cooled Argon-ion lasers with beam diameter between 1.4 and 1.7 mm |
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| 488A nm: | Small-frame, air-cooled Argon-ion lasers with beam diameter less than 0.75 mm |
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| 633 nm: | HeNe lasers, dye-head lasers, and large-frame, water-cooled Krypton-ion lasers with beam diameter of approximately 1.2 mm |
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Additional optics information:
MoFlo Optics
635 Diode Laser
For more information about our products, please contact Cytomation
at information@cytomation.com.
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