Red cell labels

G R Barclay (Robin_Barclay@compuserve.com)
Thu, 11 Sep 1997 11:08:43 -0400

Dear colleagues
I am looking for advice on labels which might be used to distinguish
damaged and/or dead red cells from healthy ones. Ideally, such a label
would readily enter healthy cells, not be quenched by haemoglobin, and be=

lost from membrane-compromised cells - and would be excitable at 488nm. =

Alternatively - it might enter cells, *not* be readily lost - and be
quenched by haemoglobin - - so that loss of haemoglobin might allow it to=

emit. Any suggestions?? I may also be looking for similar live/dead
markers for platelets - no haemoglobin, but any suggestions and pitfalls?=

Robin Barclay
Scottish National Blood Transfusion Service
Edinburgh