Edoardo Colombo MD
Director Flow Lab
Dept. of Pathology
Valduce Hospital
COMO , Italy
coledoco@tin.it
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From: G R Barclay <Robin_Barclay@compuserve.com>
To: cyto-inbox
Subject: Red cell labels
Date: gioved=EC 11 settembre 1997 17.08
Dear colleagues
I am looking for advice on labels which might be used to distinguish
damaged and/or dead red cells from healthy ones. Ideally, such a label
would readily enter healthy cells, not be quenched by haemoglobin, and be
lost from membrane-compromised cells - and would be excitable at 488nm.=20
Alternatively - it might enter cells, *not* be readily lost - and be
quenched by haemoglobin - - so that loss of haemoglobin might allow it to
emit. Any suggestions?? I may also be looking for similar live/dead
markers for platelets - no haemoglobin, but any suggestions and pitfalls?
Robin Barclay
Scottish National Blood Transfusion Service
Edinburgh
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