Pyronin Y
DEEKEWB@aol.com
Fri, 12 Sep 1997 00:22:46 -0400 (EDT)
One and all--
We are interested in using Pyronin Y (PY) staining of RNA to distinguish G0
phase from G1 phase hematopoietic cells. A recent publication suggested that
the concentration of PY had to be <5 micrograms/mL to maintain viability but
that at concentrations of about 1 ug/ml, PY preferentially stained
mitochondria. Does anyone have experience with this stain? Can they
recommend a reliable PY concentration for staining RNA "specifically" ? What
incubation period and temperature are used for PY entry? Does PY have any
effect on cell physiology at "nontoxic" concentrations? Thanks for the
advice!!
Doug Dooley
DEEKEWB@AOL.COM
American Red Cross Stem Cell Lab
Portland OR
503-280-1442