Anna Porwit-MacDonald
Haematopathology Lab.,
Karolinska Hospital, Stockholm
>
>In order to release most of the marrow cells from possible bone marrow
>particles we have for years used repeated aspiration of the aspirate through
>a small gauge needle (25 ga.). This seems to work fine, does not appear
>to damage the cells much and is fairly rapid. It is however not a very
>safe practice. I would like to stop having the people in the lab have to
>use these needles while manipulating the patient bone marrow in the lab.
>Does anyone have any better procedures for releasing cells in the
>particles?
>We use the frosted ends of microscope slides for tissues and this works
>fine but is impractical for aspirates.
>
>Tom McHugh
>Dept. Lab. Medicine
>UCSF
>mchugh@labmed.ucsf.edu
>
>