Re: bone marrow particles

Pilar Calo (calomata@facstaff.wisc.edu)
Thu, 21 Aug 1997 12:05:12 -0500

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Tom,
we get the bone marrow aspirates diluted 1:1 with phys.NaCl + heparine , and
before taking from the aspirate for staining we put it on shaker for a very
short while. We did not see differences in numbers of cells in various
populations in different tubes so it seems that this is enough for routine
examinations .
Best wishes
Anna

Anna Porwit-MacDonald
Haematopathology Lab.,
Karolinska Hospital, Stockholm

>
>In order to release most of the marrow cells from possible bone marrow
>particles we have for years used repeated aspiration of the aspirate through
>a small gauge needle (25 ga.). This seems to work fine, does not appear
>to damage the cells much and is fairly rapid. It is however not a very
>safe practice. I would like to stop having the people in the lab have to
>use these needles while manipulating the patient bone marrow in the lab.
>Does anyone have any better procedures for releasing cells in the
>particles?
>We use the frosted ends of microscope slides for tissues and this works
>fine but is impractical for aspirates.
>
>Tom McHugh
>Dept. Lab. Medicine
>UCSF
>mchugh@labmed.ucsf.edu
>
>

Next message: Cheryl M. A. Palmer: "Lymphocyte Populations"
Previous message: Pilar Calo: "Re: B cell precursors"
Maybe in reply to: Tom Mchugh: "bone marrow particles"
Next in thread: Rafael Nunez: "NK leukemia?"
Reply: Rafael Nunez: "NK leukemia?"