Lymphocyte Populations

Cheryl M. A. Palmer (cpalmer@uwimona.edu.jm)
Thu, 21 Aug 1997 10:31:08 -0500 (GMT-0500)

I use a FACScan along with Simulset software to enumerate T- lymphocyte
subsets on HIV positive patients. On a few occasions I have seen two
distinct lymphocyte populations. On gating these populations, whether
individually or together, the T+B+NK cells are much lesser than the
ideal 100%.
Can someone give me some ideas as to why this is happening and how I can
account for the 'missing' cells.

Thank you .
Cheryl Palmer