bone marrow particles
Tom Mchugh (mchugh@pangloss.ucsf.EDU)
Mon, 11 Aug 1997 17:20:23 -0700 (PDT)
In order to release most of the marrow cells from possible bone marrow
particles we have for years used repeated aspiration of the aspirate through
a small gauge needle (25 ga.). This seems to work fine, does not appear
to damage the cells much and is fairly rapid. It is however not a very
safe practice. I would like to stop having the people in the lab have to
use these needles while manipulating the patient bone marrow in the lab.
Does anyone have any better procedures for releasing cells in the
particles?
We use the frosted ends of microscope slides for tissues and this works
fine but is impractical for aspirates.
Tom McHugh
Dept. Lab. Medicine
UCSF
mchugh@labmed.ucsf.edu