CellProbe™ Reagents
Whole Blood To Histograms
In Minutes
CellProbe substrates are "flow-ready" reagents and are designed for rapid preparation and analysis. For whole blood applications, users can take advantage of the superior quality, speed and ease-of-use of the COULTER® Q-Prep Workstations and Reagent System. And with the short incubation steps users can process a blood sample and be analyzing their cytometry results in less than 45 minutes. In comparison, traditional enzyme analysis methods can take several hours to several days to complete.

These reagents can be used with a wide variety of samples that provide single cell suspensions including blood and body fluids, mononuclear cell preparations, cultured cells and disaggregated tissue cells -- virtually any type of sample that is currently being used in flow cytometry. In addition, CellZymeTM cell control verifies reactivity for all CellProbe substrates.

CellProbe FDA, NaF•Esterase levels measured in Kawasaki Disease cells treated with intravenous gammaglobulin. Measurements were done on normal donor cells, Kawasaki cells pretreatment, and days 4, 8, and 12 post-treatment. Results indicate that altered enzyme activity levels of FDA, NaF•Esterase appear to resolve toward levels detected in normal samples within 8 days after treatment. (Courtesy of D. Kinney, Center for Blood Research, Boston, MA).

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu