Flow Cytoenzymology
Research Applications
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CellProbe™ Reagents Flow Cytoenzymology Research Applications |
Opening A Vast Array Of Research Opportunities
Since enzymes are involved in every cell type and virtually every cellular function and process, the CellProbe line can be used in an extensive number of research applications. Their ability to detect small changes in enzyme activity and concentration makes these substrates a powerful adjunct to your current flow cytometry research endeavors, opening new areas of study that were previously beyond your reach. And CellProbe reagents can even be used in conjunction with your current monoclonal antibodies to measure intracellular enzymes in a particular subset via simultaneous analysis of two or more colors.
| Flow Cytoenzymology Research Applications | |
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| Cell Identification | Blast Cells, Nucleated RBC's, T/B Cell |
| Cell Lineage | Myeloid, Lymphoid |
| Cellular Processes | Activation, Apoptosis, Signal Transduction, Maturation |
| Cell-Cell Interaction | Antigen Presentation, Cell Cytotoxicity, Cell Activation |
| Host Defense Mechanisms | Oxidative Burst, Phagocytosis, Inflammation |
| Cell Migration | Tumor Metastasis, Inflammation |
| Drug Effects on Cells | e.g. Kawasaki Disease and Steroid Modulation in JRA |
| Disease Related Cellular Research | HIV, Leukemia/Lymphoma, Transplantation, Autoimmunity |
| Basic Research in Cell Biology | Cell Function, Cellular Responses, Cytokine Production |
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| Two peripheral whole blood samples stained with CellProbe GP•DPP IV. The histogram at left shows virtually no nucleated red cells (NRBC's). The histogram at right is from a high NRBC sample. NRBC's exhibit low DPP IV activity in comparison to leukocytes. (Courtesy of N. Kuylen, N. Garcia, Coulter Corporation). |
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| Ungated histogram of two-color experiment with CD8-PE and CellProbe GP•DPP IV. Sample is lysed whole blood from a normal donor. Color coded populations are: lymphocytes (green), monocytes (blue), granulocytes (red).(Courtesy of P. Ruiz, University of Miami School of Medicine, Miami, FL). |
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| Overlay of peripheral blood granulocytes from a normal donor and from a leukemic sample. Both were stained with CellProbe L•Aminopeptidase, prepared with Q-Prep, and run on an XL System. (Courtesy of A. Galiounghi, Coulter Corporation). |
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CellProbe Reagents - The Inside Story Whole Blood To Histograms In Minutes The Leader In Cell Analysis Reagent List Slide Presentation |
*For Research Use Only. Not For Use In Diagnostic Procedures.
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