Re: sorting of chromosomes

Gerald E. Marti (gemarti@helix.nih.gov)
Mon, 28 Oct 1996 08:39:43 -0500 (EST)

I would like to add anoather question concerning chromosome sorting. Is
there a second dye that can be paired with propidium iodine for two color
and be excited with a 488 laser? Or two other dyes that bind DNA and can
be excited at 488? Would like to avoid uv excitation if possible. Thank
you.

Hello
>I want to start sorting of chromosomes with
>an Epics Elite ESP from Coulter. Who has
>experience with sorting of chromosomes with
>the Coulter maschine. How difficult is it?
>As I have no experience with sorting of
>chromosomes, I would be glad to get any
>information about the sorter setup ...
>The sorter configuration is:
>Epics Elite ESP
>15 mW 488 nm Argon laser
>20 mM 325 nm HeCd laser.
>Gated Amp.
>
>Thanks
>
>Andreas
>
>
>------------------------------------------
>
>Andreas Simm
>Physiologische Chemie II
>Biozentrum, am Hubland
>D-97074 Wuerzburg
>
>FAX: +49-931-888-4113
>Tel: +49-931-888-4128
>
>E-Mail: Simm@biozentrum.uni-wuerzburg.de
>
>----------------------------------------

Gerald E. Marti
Flow and Image Cytometry Section
Laboratory of Medical and Molecular Genetics
Division of Cell and Gene Therapies
CBER FDA NIH Bdg 29B Rm 502
8800 Rockville Pike
Bethesda,MD 20892
301-827-0453 voice
301-827-0449 fax

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