Re: Calculating CD4:CD8 ratios

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I would suggest you use CD3 instead, there is a Macaque CD3 available from
Biosource (ph 1 800 242 0607) works fine. Using CD2 can lead to misleading
results as NKs which can make up a considerable and variable proportion of
your cells stain for CD2 but not CD3.

As for ratios I would use %CD3+CD4+ : %CD3+CD8+

Dunno what to do about the double positives

Simon

PS Mr Biosource you must owe me a bottle of Scotch this Christmas!

Simon Monard
FACS Lab Manager
Aaron Diamond Center for AIDS Research
455 First Avenue
New York
NY 10016

Ph (212) 448 5188
FX (212) 725 1126

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu