Calculating CD4:CD8 ratios

• Messages sorted by: [ date ][ thread ][ subject ][ author ]
• Next message: Alice L. Givan: "indo beads"
• Previous message: monard@adarc.nyu.edu: "Re: F.Y.I."
• Next in thread: monard@adarc.nyu.edu: "Re: Calculating CD4:CD8 ratios"
• Maybe reply: monard@adarc.nyu.edu: "Re: Calculating CD4:CD8 ratios"

In a tube which combines CD2(FITC)/CD4(PE)/CD8(PerCP) mAb's, which
values are most appropriate to use to calculate the CD4:CD8 ratios
correctly. Would one use:

(i) the total %CD4 and the total %CD8 derived from single parameter
histograms?
or

(ii) the %CD4+CD8- and the %CD8+CD4- derived from the CD4 vs CD8 dot
plot quadrants?
or

(iii) Should one be using the %CD2+CD4+ and the %CD2+CD8+ values
(Something that was just recently suggested to me)?


Thanks for all replies/insights in advance.
Diane Ladouceur
(613-957-0998)

NB/ We were using CD2 instead of CD3, as this work is being done on
Cynomolgus monkeys, and at the time we had not yet identified a CD3 mAb
which cross-reacted with this species.

• Next message: Alice L. Givan: "indo beads"
• Previous message: monard@adarc.nyu.edu: "Re: F.Y.I."
• Next in thread: monard@adarc.nyu.edu: "Re: Calculating CD4:CD8 ratios"
• Maybe reply: monard@adarc.nyu.edu: "Re: Calculating CD4:CD8 ratios"

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu