Re: saturation binding & fluorescence intensity
Thomas Delohery (t-delohery@ski.mskcc.org)
Wed, 16 Oct 1996 16:39:44 +0530
>This is not true... fluorescence intensity is ALWAYS proportional to the
>antigen
>density. The problem at sub-saturating amounts of antibody is that it is also
>proportional (roughly) to antibody concentration as well as the time of the
>stain! We have done quantitative antigen density measurements with antibodies
>at subsaturating concentrations. However, to do so, we needed to ensure that
>(1) the staining volume was precisely controlled; (2) the amount of
>antibody was
>precisely controlled; and (3) the timing of the incubation (and volume of
>subsequent washes, for that matter) was controlled.
>
>No arguments here, Tom, just the facts.
>
>mr
Thanks for the clarification. BTW, that wasn't the part of my post where I
expected an argument.
td
--
==============================================================================
Thomas Delohery | Internet: t-delohery@ski.mskcc.org
Manager, Flow Cytometry Core Facility | Phone: (212) 639-8729
Memorial Sloan-Kettering Cancer Center | Fax: (212) 794-4019
1275 York Ave. Box 98 |
New York, NY 10021 |
==============================================================================
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
