Hoechst; Viable sorting - cell cycle..question?
Faust, J. S. (jsfaust@wista.wistar.upenn.edu)
Thu, 10 Oct 1996 11:01:07 -0400
I have a user who would like to sort for live cells based on position in
cell cycle. Does anyone have experience, a staining protocol, filter setup,
hints, or tricks for using Hoechst for this purpose. I will be using a
Coulter Elite, separated dual beam alignment, and a HeCd for excitation of
the Hoechst. Thanks.
Jeffrey S. Faust
Philadelphia, PA
215-898-3811 (work)
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