Re: PE/Cy5 on a FACS Calibur

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1. The FACSCaliber with 2nd laser (diode) uses a 670nm LP in FL3, so
all the PE/CY5 reagents work great. In fact, we've also had good success with
red 613 getting into the FL3 at > 670nm (default FACSCan/single laser is 650nmLP).
The longer wavelength BP filter in FL3 is needed to block the 633nm diode laser.

2. The FACSCaliber electronics allow for intralaser compensation. Our
users are generating rather spectacular data using combos like FITC, PE, PE/CY5,
and APC. The FL3/FL4 compensation is straight forward.

Regards,

Joe

• Next message: Joseph Webster: "Re: Phenol red fluoresecence?"
• Previous message: Derek Davies: "3rd colour out of 4"
• Maybe in reply to: Calman Prussin: "PE/Cy5 on a FACS Calibur"
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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu