Re: Phenol red fluoresecence?

Joseph Webster (J.Webster@centenary.usyd.edu.AU)
Wed, 25 Sep 1996 09:08:40 +1100

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At 12:16 PM 24/09/96 EDT, Robin Barclay wrote:
>
>Does anyone know whether phenol red, the common pH indicator in tissue culture
>medium, has any fluorescent properties when associated with cells or any
>quenching properties for other fluorochromes?

I don't know about quenching, but phenol red certainly does fluoresce in
all channels on a FACScan.
At the very least it gives high backgrounds, sometimes makes the readings
completely useless.

Avoid it if possible!

Cheers, Joseph.
Joseph Webster (O.I.C. Flow Cytometry)
Centenary Institute of cancer Medicine & Cell Biology
Locked Bag No.6, Newtown, NSW 2042, AUSTRALIA.
Ph: 61-2-9565-6110 Fax: 61-2-9565-6101

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu