3rd colour out of 4

Derek Davies (davies@icrf.icnet.uk)
Wed, 25 Sep 1996 13:46:20 +0100 (BST)

There has been some discussion on this list about the third colour to be
used in a 4 colour surface-labelling experiment. If one is using a He-Ne
as the second laser presumably there could be problems with using a
tandem dye where the second half of the tandem is Cy5 or APC or whatever
that will be excited by some degree by the second laser.

Using a non-tandem dye such as BD'd PerCP can get round that problem, but
it is well-documented that PerCP is photobleached at high laser powers
that are used on sorters.

The reason I am writing all this is that today I have received some
literature about a dye called ALPHA-RED which comes from Exalpha
Laboratories in the States (Through SeroTec here in the UK). I have been
told that this is not a tandem dye and that it is excited at 488nm and
emits at 670nm so it is obviously a possible alternative to PerCP.

Has anybody out there heard of this dye or used it? Any comments?

Derek

Derek Davies
FACS Laboratory,
Imperial Cancer Research Fund, London, UK

http://www.icnet.uk/axp/facs/davies/index.html

Simon wrote:

>>I too would like to be illuminated as to which dyes can be used for four
>>colour work with the Calibur, are we really stuck with PerCP as the only
>>third colour?

>>Simon

>>Simon Monard
>>FACS Lab Manager
>>Aaron Diamond Center for AIDS Research

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